Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human ApoB Detection Kit Standard
HTRF Human ApoB Detection Kit Standard
Revvity Health Sciences Inc HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 1,000 Assay Points
HTRF CD16a (FcgRIIIa, F158) Cellular Binding Kit, 1,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total SALL4 Detection Kit, 10,000 Assay Points
HTRF Human Total SALL4 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total Cofilin Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total Cofilin Detection Kit, 50,000 Assay Points
Molecular Devices Filter Lens, 450 nm, For Microplate Readers
The Molecular Devices 450 nm filter lens (MPN 6500-0007) is an optical replacement filter designed for compatible microplate readers. It provides a center wavelength of 450 nm for use in fluorescence and absorbance detection applications and serves as an interchangeable detection filter for select filter-based plate readers.
- Provides center wavelength of 450 nm.
- Intended for fluorescence and absorbance detection.
- Replacement optical filter lens for compatible microplate readers.
- Compatible with select filter-based plate reader models.
Revvity Health Sciences Inc HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Revvity Health Sciences Inc HTRF Human Total FGFR4 Detection Kit, Control Lysate
HTRF Human Total FGFR4 Detection Kit, Control Lysate