Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK9 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total CDK9 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-MEK1 (Ser298) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MEK1 (Ser298) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total FLT3 Detection Kit, 500 Assay Points
HTRF Human Total FLT3 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Total Myd88 Detection Kit, 10,000 Assay Points
HTRF Total Myd88 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 10,000 Assay Points
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Duolink(R) In Situ Wash Buffers, Fluorescence | 20L
Revvity Health Sciences Inc HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, Control Lysate
HTRF Human Phospho-Cyclin D1 (Thr286) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Ataxin 2 Mutant Detection Kit, 500 Assay Points
HTRF Human Ataxin 2 Mutant Detection Kit, 500 Assay Points
Molecular Devices Microplate Reader, 6-1536-Well, Dual PMT, User-Upgradeable
The SpectraMax Paradigm multi-mode microplate reader from Molecular Devices is a user-upgradeable detection platform for absorbance, fluorescence, time-resolved fluorescence, fluorescence polarization, and luminescence assays. It supports 6- to 1536-well plates and modular detection cartridges for configurable optics.
- User-upgradeable detection cartridges for configurable optics and modes.
- Supports 6- to 1536-well plate formats.
- Dual photomultiplier tubes for enhanced sensitivity and throughput.
- Wide absorbance range 230-1000 nm and fluorescence excitation/emission coverage.
- Integrated temperature control, plate shaking, and robotic compatibility.
- High dynamic range greater than six decades for quantitative assays.
- Compact footprint: 39 cm W x 45 cm H x 63 cm D.
